Date of Award
Master of Science
Amy de Jongh Curry
Acute respiratory distress syndrome patients are usually administered high levels of oxygen and then mechanical ventilation. The combination of these is thought to cause worse lung injury than either alone through biophysical mechanisms partly involving overdistension. The basis of this thesis is that injury may be associated with degradation of hyaluronan, a key component of the exracellular matrix having compressive resistance. Although hyaluronan is known to degrade directly by reactive oxygen species or by hyaluronidase, it is possible to measure the contribution of hyaluronan degradation in lung structure. Molecular weight measurements were used to confirm hyaluronan degradation, while nano indentation of commercially available hyaluronan hydrogel was used to measure the stiffness changes due to degradation. Bacterial hyaluronan degradedwith hydrogen peroxide and hyaluronidase; both treatments led to changes in the stiffness of the hydrogel. In summary, this hydrogel may serve as an in vitro model of hyaluronan degradation in the lungs.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Komolafe, Adetoun Opetola, "Contribution of Hyaluronan Degradation on the Structure of Lungs: Development of Experimental Techniques" (2014). Electronic Theses and Dissertations. 1048.