Date of Award
Master of Public Health
Wilfried Joachim Jurgen Karmaus
ProblemImmune changes occur during pregnancy, including a bias towards Th2 responses. This study explored maternal DNA methylation (DNA-M) of Th1, Th2, Th17, and Treg pathway genes before and during pregnancy.MethodsBlood DNA-M was measured in 54 women at age 18, and again in the first (weeks 8-21, n=39) and second (weeks 22-38, n=35) halves of pregnancy. Cytosine-phosphate-guanine (CpG) sites in genes of the T cell pathways were compared to random samples of CpGs, contrasting non-pregnancy with first and second halves of gestation using mixed linear models.ResultsMore CpGs within the Th2 pathway (35%) changed significantly compared to randomly selected CpGs (24%) in the first half. In second half, more CpGs of Th1 (36%) and Th2 (34%) pathways changed significantly.ConclusionsMethylation changes in the Th2 pathway genes suggest involvement of DNA-M in the Th2 bias. Changes in the Th1 pathway need further exploration.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Iqbal, Sabrina, "Changes in DNA methylation of CpGs from age 18 to pregnancy in Th1, Th2, Th17, and Treg pathway genes" (2015). Electronic Theses and Dissertations. 1297.