Date of Award
Master of Science
Health and Sport Science
Marie van der Merwe
Thomas R Sutter
Visceral adipose tissue (VAT) physiology is negatively affected by chronic glucocorticoid (GC) usage, and is exacerbated by a "Western" diet. However, the impact of omega-3 supplimentation into a "Western" diet, during chronic GC usage, remains unknown. Therefore, we determined the impact of both diets ("Western" vs omega-3 supplimented) in conjunction with chronic GCs, on VAT physiology. Sixty-four male C57BL/6 mice (n=8-16/group) were subjected to 4-weeks of dietary intervention (high fat lard [HFL] vs. high fat fish oil [HFO], with or without prednisolone [40mg/kg/m2] daily). We hypothesized that omega-3 supplimentation would protect VAT physiology from chronic GC-induced negative effects. Overall, both HFO groups gained less body weight, displayed less VAT and smaller adipocytes, retained a greater percentage of M2-polarized macrophages, and exhibited beneficial alterations in gene expression as compared to both HFL groups. Our data indicate that VAT physiology is protected by an increase in dietary omega-3s, irrispective of GC usage.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Hill, Jessica Lynn, "Glucocorticoids Induce Molecular and Phenotypical Changes in Visceral Adipose Tissue When Consuming a High Fat Diet" (2017). Electronic Theses and Dissertations. 1592.