Date of Award
Master of Science
Health and Sport Science
Marie van der Merwe
The gastrointestinal tract (GIT) adapts in response to dietary intake and glucocorticoid dosing through altered villus length, crypt depth, and numbers of proliferating intestinal stem cells (ISC). The goal was to characterize the impact of glucocorticoids in combination with different high fat diets on the GIT. Seventy-two juvenile male C57BL/6 mice (n=2-10/group) were subjected to 4-weeks of dietary intervention (high-fat lard [HFL] vs. high-fat fish-oil [HFO], with or without prednisolone [40mg/m2/kg] daily). HFO mice had increased villi length in the duodenum and jejunum while the villi in the ileum were the same across all diets (Chow, HFO, and HFL). Additionally, both high fat diets had decreased crypt depth in the jejunum and ileum correlating decreased Ki-67 staining within the crypts. Our data indicates that HFO fed mice are more sensitive to glucocorticoids. Caution should be taken when consuming a HFO diet in conjunction with high doses of glucocorticoids.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Wyman, Jacqueline Marie, "Dietary Fatty Acid Composition During Glucocorticoid Treatment: Implications for the Gastrointestinal Tract" (2017). Electronic Theses and Dissertations. 1596.