Master of Science
Jessica Amber Jennings
Implanted materials can increase the risk of osteomyelitis, leading S. aureus to develop a biofilm not only on the materials, but also in bone and soft tissue surrounding the joint. Biofilm is intrinsically less susceptible to antibiotic therapy than free-floating planktonic microorganisms due to decrease metabolic rates of persister cells. Mannitol has been shown to active persister cell metabolism, priming mircroorganisms for the uptake of antibiotics and subsequent eradication. Blends of mannitol and chitosan were evaluated in elution and activity studies to determine the efficacy against biofilm with additional injectability, degradation, and biofilm eradication evaluations Results indicate the mannitol/chitosan blend is capable of eluting antibiotics for up to 7 days and antimicrobial activity up to 7 days. Clincially this paste could serve as a biodegradable local antibiotic delivery system at the time of surgery to prevent infection, during periprosthetic joint surgeries, or complex musculoskeletal trauam to prevent and treat infection.
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Pace, Leslie Rae, "Development and Evaluation of an Injectable Chitosan-Mannitol Paste as an antimicrobial delivery system for the inhibition and eradication of biofilm" (2019). Electronic Theses and Dissertations. 2002.