Electronic Theses and Dissertations

Identifier

6501

Date

2019

Document Type

Thesis

Degree Name

Master of Science

Major

Biomedical Engineering

Committee Chair

Joel Bumgardner

Committee Member

Jessica Amber Jennings

Committee Member

Judith Cole

Abstract

This study evaluated the potential of adding raspberry ketone (RK) to electrospun chitosan membranes (ESCMs) to create a bioactive guided bone regeneration GBR) membrane capable of stimulating bone cell differentiation. In this study, W-20-17 cells exposed to 50-200μg/ml RK showed an increased expression of alkaline phosphatase (ALP). RK was loaded onto ESCM discs modified by one of three different fatty acid anhydrides and release profiles were examined. It was found that the RK release profile was dependent on the type of fatty acid treatment used. RK loaded membranes were then evaluated for cytocompatibility and osteodiferentiation potential. While the RK released from the membranes had no cytotoxic effect on the cells, it id not induce osteodifferentiation. It is speculated that RK levels were not sustained at high enough concentrations to affect the cells. ESCMs, with a more sustained RK release profile, may have potential to be used in GBR applications.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.

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