Master of Science
Jessica A Jennings
Wounds resulting from musculoskeletal trauma cause pain and often infection of damaged tissue. Up to 80% of these infections are due to biofilm formation on wounded tissue. Bacteria within a biofilm can withstand 1000X the minimum inhibitory concentration of antibiotics, demonstrating the need for new therapeutics to prevent and treat these infections. Cis-2-decenoic acid (C2DA) disperses biofilms and can prevent biofilm formation of many microorganisms. Additionally, local anesthetics like bupivacaine have antimicrobial effects against bacteria. This study sought to evaluate modified electrospun chitosan membranes as wound dressings that release C2DA and bupivacaine to prevent infection and alleviate pain associated with wounds. Release profiles of therapeutics were evaluated, with results indicating that membranes release active concentrations of both therapeutics for 72 hours. Though higher concentrations of bupivacaine were not cytocompatible with fibroblasts, membranes were effective in preventing Methicillin-resistant Staphylococcus aureus biofilm growth in vitro.
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Harrison, Zoe Lynn, "In Vitro Evaluation of Loaded Chitosan Membranes for Pain Relief and Infection Prevention" (2020). Electronic Theses and Dissertations. 2117.