Date of Award
Master of Science
Amy de Jongh Curry
Most amputees experience phantom limb pain (PLP) post-amputation, the mechanisms of which are not fully understood. Rat amputees may serve as a practical model for studying PLP, as rats process pain similarly to primates. Ultrasonic vocalizations (USVs) of acclimated, formalin-injected rats and acclimated, forelimb-amputated rats were recorded to investigate the potential for measuring USVs as an indicator of a negative affective state. Subcutaneous injections of 10% formalin in the hindpaw elicited increased USV emission, primarily in the 22 kHz-AFR. Unoperated rats and forelimb-amputated rats were monitored for seven weeks following acclimation and surgery. Amputees emitted a greater quantity of 22 kHz-AFR USVs than controls at 4-7 weeks post-amputation and a greater duration at 4-6 weeks; however, there were no differences in weeks 1-3. These differences in USV behavior may indicate a difference in affective state between groups. Further studies of USVs in forelimb-amputated rats should incorporate additional behavioral measures of pain.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Womack, Carey Daniel, "Ultrasonic Vocalizations as a Potential Indicator of a Negative Affective State in Forelimb-Amputated Rats" (2020). Electronic Theses and Dissertations. 2118.