Date of Award
Master of Science
Evidence supports that osteoarthritis (OA)has a chronic low-grade inflammatory state that contributes to the progressive loss of articular cartilage. Reactive oxygen species (ROS)are elevated following injury and mediate inflammation. Cellular therapy using mesenchymal stem cells (MSCs) induce anti-inflammatory and healing responses in native tissue, but efficacy and reproducibility are lacking. Since OA treatments involving MSCs introduce cells into a damaged and inflammed joint, excess oxidative stress could damage these cells reducing their benefit. Cytokine-stimulated chondrocytes treated with hydrogen peroxide scavenging poly-propylene sulfide microspheres(PPS-MS) showed reduced expression of collagenase (MMP-13) with a narrow window of efficacy. Co-culture investigation with MSCs showed no reduction of MMP-13 expression nor increase in expression of anabolic factors like type IIcollagen. Pretreatment with PPS-MS prior to the addition of MSC therapy did not provide conclusive evidence for improved anabolic to catabolic gene expression ratios over a five-day culturing period.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Pasternak, Zachary Alexander, "In Vitro Analysis of Antioxidant Poly(Propylene Sulfide)-Microspheres for Pretreatment of Inflammatory Cytokine-Induced Chondrocytes to Facilitate Adipose-Drived Stem Cell Therapy" (2021). Electronic Theses and Dissertations. 2170.