Date of Award
Thesis (Access Restricted)
Master of Public Health
Wilfried JJ Karmaus
This proof of concept study, focusing on the IL1RL1 gene as example, examines whether maternal asthma links grand maternal asthma to wheezing in grandchildren via DNA methylation (DNA-M) of this asthma associated gene. We analyzed a three generational (F0, F1, F2) cohort from the Isle of Wight, UK. DNA-M was determined in peripheral blood from 18 year old F1 women (n = 245) and in umbilical blood from F2 children (n = 129). F0 maternal asthma decreased DNA-M of cg17738684 in F1 daughters (p = 0.025). Lower methylation at this CpG increased the relative risk (RR) of asthma in the F1 (p < 0.001) and was related to lung function. In turn, F1 maternal asthma reduced methylation of cg17738684 in the F2 (p = 0.024), which then elevated the risk of infant wheeze (p < 0.007). F1 anf F2 methylation levels were not correlated. Non-genetic mechanisms of intergenerational transmission of asthma need intauterine exposure to maternal and adaptive DNA-M.
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Tetali, Sabarinath Reddy, "A Role For IL1RL1 in Intergenerational Epigenetic Transmission of Asthma in Three Generations of The Isle of Wight Birth Cohort" (2015). Electronic Theses and Dissertations. 2214.