Date of Award
Thesis (Access Restricted)
Master of Public Health
Wilfried J. J. Karmaus
Lung function is a marker of asthma. We hypothesize that differential DNA methylation (DNA-M) of cytosine-phosphate-guanine (CpG) sites in early life can predict lung function in later childhood and adolescence. We analyzed 274,709 CpG sites with lung function in two prediction-periods (PPs) on girls in Isle of Wight (IOW) cohort: neonatal DNA-M to lung function at age 10 (prediction-period 1; PP1) and DNA-M at age 10 to lung function at 18 (prediction-period 2; PP2) using a screening technique and corroborated the findings with linear regression. Significant CpG sites associated with lung function in same direction at both PPs were replicated in Swedish BAMSE cohort. Three CpG sites in PP1 and 19 in PP2 are statistically significant with similar effects in both periods. Based on replication analyses of 22 CpG sites, six are associated with lung function in same direction with IOW and three of six sites have statistically significant effects.
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Sunny, Shadia Khan, "Differentially Methylated CpG Sites in Early Life May Predict Lung Function in Childhood and Adolescence: A Genome-wide Approach" (2016). Electronic Theses and Dissertations. 2237.