Date of Award
Master of Science
Marie van der Merwe
Background: Obesity and MetS has been shown to negatively impact protein synthesis rates. Objective: Determine the effects of sex and high fat/high sugar diets on mTOR signaling pathways. Methods: 15 male and 15 female wild type C57BL/6 mice were randomized into 3 groups: High Fat (45% fat), High Sugar (60% carb) and Control. While continuing diets, body weight and body composition were monitored. Following sacrifice, muscles were examined for changes in the mTOR signaling pathway. Results: Mice consuming the HF diet had the highest levels of weight gain. Females expressed higher levels of expression of P-AMPK. Levels of REDD1 were significantly higher in females than males with no effect of diet. Conclusion: There was a sex difference in body weight and muscle mass. Sex had an effect on 4EBP1 but not S6 levels.
Dissertation or thesis originally submitted to ProQuest
Powell, Zereque, "Effect of High Fat vs High Sugar Diet on mTor Signaling Pathway in Skeletal Muscle of Male and Female Mice" (2023). Electronic Theses and Dissertations. 3112.