Date of Award
Master of Science
Warren O. Haggard
Richard A. Smith
Due to limitations of bone autografts and allografts there is much research directed at designing synthetic bone grafts. In this work, collagen-chitosan-calcium phosphate microparticles and microparticle-based scaffolds were compared to their counterparts without collagen in terms of degradation, cytocompatibility (porosity and stiffness only for scaffolds). Microparticles exhibited 20% decrease in mass over 6 weeks and provided an optimal environment for 3-5 fold cell proliferation over 7 days-culture period. Although there was no effect of collagen addition to microparticles, all the formulations may be suitable as bone tissue fillers. Further, there was no difference between control and collagen scaffolds. In general, scaffolds exhibited 23% porosity, 0.6-1.2 MPa Young's modulus, 10-25% degradation over 4 weeks, and supported a 4-7-fold increase in osteoblast cell number over 7 days in culture. While there is room to improve Young's modulus of the scaffolds, they are satisfactory bone graft substitutes.
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Zugravu, Monica Viorica, "Design and Biophysicochemical Properties of Chitosan-Collagen-Calcium Phosphate Microparticles and Scaffolds for Bone Tissue Regeneration" (2012). Electronic Theses and Dissertations. 426.