Date of Award
Master of Science
Joel D Bumgardner
Warren O Haggard
Richard A Smith
John L Williams
Goodhart, John Matthew. M.S. The University of Memphis. August 2012. Design and Validation of a Cyclic Strain Bioreactor to Condition Spatially-Selective Scaffolds in Dual Strain Regimes. Major Professor: Dr. Joel Bumgardner. The objective of this study was to design and validate a unique bioreactor design for applying spatially-selective, linear, cyclic strain to degradable polymeric fabric scaffolds. Image analysis of polyethylene terephthalate (PET) scaffolds subjected to 3% mechanical stretch showed that the stretched and unstretched portions of the scaffolds experienced 2.97±0.13% and 0.02±0.18% strain respectively. NIH-3T3 fibroblast cells were cultured on PET scaffolds and half of each scaffold was stretched 5% at 0.5 Hz for one hour per day for 14 days in the bioreactor. Scaffolds were assayed for cell viability and proliferation as well as glycosaminoglycan (GAG) and hydroxyproline concentration. Bioreactor scaffolds showed a 700% and 75% increase in DNA and hydroxyproline concentration respectively as well as a significant decrease in GAG concentration over the control scaffolds. Surprisingly, little differences were seen between stretched and unstretched portions of the scaffolds likely due to the conditioned media effect.
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Goodhart, John Matthew, "Design and Validation of a Cyclic Strain Bioreactor to Condition Spatially-Selective Scaffolds in Dual Strain Regimes" (2012). Electronic Theses and Dissertations. 574.