Date of Award
Master of Science
Charles A Lessman
Judith A Cole
Bill A Simco
Kaushik, Gayathri. M.S. The University of Memphis. August/2013. Ovarian dynamics in zebrafish (Danio rerio): A study of growth, vascularization, and estrogenic effects in follicle-enclosed oocytes in vivo. Major Professor: Dr. Charles A. LessmanThe timeline of follicle-enclosed oocyte growth and development is not known. To study this, mature female zebrafish, Danio rerio, were injected intraperitoneally with the marker trypan blue (TB). Ovaries were removed at different time post injection. Then, oocyte diameter and TB uptake were determined from brightfield and fluorescence microscopy images, respectively. TB uptake increased steadily up to 48 hours post injection and declined subsequently. In Tg(fli1:EGFP)y1 zebrafish that express green fluorescent protein in endothelial cells, ovarian blood vessel diameter was greater in larger oocytes and unaffected by in vitro treatments with vasoactive agents. In the transparent zebrafish, casper, oocytes naturally cycled between growth and regression in vivo. Exogenous estradiol and tamoxifen both disrupted the cycle and maintained ovaries in the regressed phase. Surprisingly, stopping the hormone treatment, restored the natural cycle. Together, these studies provide the foundation and tools for future experiments on oocyte development in zebrafish.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Kaushik, Gayathri, "Ovarian Dynamics in Zebrafish (Danio rerio): A Study of Growth, Vascularization, and Estrogenic Effects in Follicle-Enclosed Oocytes in vivo" (2013). Electronic Theses and Dissertations. 788.