Electronic Theses and Dissertations

Identifier

1080

Hengjie Su

Date

2014

Document Type

Thesis

Degree Name

Master of Science

Major

Biomedical Engineering

Committee Member

Joel Bumgardner

Committee Member

Eugene Eckstein

Committee Member

Warren Haggard

Abstract

Guided bone regeneration (GBR) membranes function as barriers in preventing soft tissue migration into dental osteogenic spaces. Chitosan is a biocompatible and degradable natural polysaccharide, which has shown potential in tissue regeneration and GBR applications. Trifluoroacetic acid (TFA) is a common solvent used to make the chitosan polymer solution for electrospinning process. However, the TFA salts remaining in the electrospun nano-sized fibers induce the chitosan fiber swelling and dissolution when placed in aqueous solutions leading to loss of nano-fiber structure. The aim of this work was to evaluate an innovative method using triethylamine (TEA)/ di-tert-butyl dicarbonate (tBoc) to treat the electrospun chitosan membrane without losing nanofibrous structure or compromising compatibility. After the treatment, the nanofibrous structure was well maintained in water overnight, which was confirmed by the SEM. FTIR analyses confirmed TFA salt removal. Surgical fixation tests indicated TEA/tBoc membranes have appropriate clinical handability. The TEA/tBoc treated membrane also showed good cytocompatibility with osteoblastic cells, as well as suitable degradation ration (23.7%) after 7 weeks.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.

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