Subcellular localization of pesticides


Although apparently successful for localization of insecticides in insect nerves, high resolution autoradiography cold not be adapted for a similar study of mouse tissues. The penetration and/or binding of insecticides may be different between the two animal groups, although concentration of the radioactive label in the smaller organisms might also be a partial explanation. The extent of leaching during fixation and embedding processes was examined with treated LM strain mouse fibroblast culture cells, and important differences in retention of label among different preparative methods were found. High resolution autoradiography has limited value in studying the localization of fat soluble pesticides in mammalian tissue until routine procedures are developed that eliminate the necessity for fixing and dehydrating tissue in preparation for electron microscopy. (Journal received: Feb. 1975)

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