Immobilization of fibrinogen antibody on self-assembled gold monolayers for immunosensor applications
Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.
Tissue Engineering and Regenerative Medicine
Cho, H., Zook, J., Banner, T., Park, S., Min, B., Hasty, K., Pinkhassik, E., & Lindner, E. (2014). Immobilization of fibrinogen antibody on self-assembled gold monolayers for immunosensor applications. Tissue Engineering and Regenerative Medicine, 11 (1), 10-15. https://doi.org/10.1007/s13770-013-1119-4