Image reconstruction for three-dimensional transmitted-light DIC microscopy


The derivation of an iterative method for phase reconstruction from differential-interference-contrast (DIC) images is presented here. Because DIC imaging is direction sensitive, in our approach we estimate a specimen's phase function using multiple DIC images obtained by rotating the specimen. Results obtained from testing the method via two- dimensional simulations demonstrate that the use of multiple DIC images at different specimen rotations yield phase reconstructions that more closely resemble the phase function of phantoms than the unprocessed DIC images. Improvement in resolution was also achieved: two points separated by half the Rayleigh resolution limit for coherent illumination not resolved in the unprocessed DIC image, were successfully resolved in the reconstructed phase images. Our results show that phase reconstructions are quantitatively better and resolution is improved when two or more DIC images are used in the reconstruction. ©2004 Copyright SPIE - The International Society for Optical Engineering.

Publication Title

Proceedings of SPIE - The International Society for Optical Engineering