Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy


Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and threedimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions. © 1999 Optical Society of America.

Publication Title

Journal of the Optical Society of America A: Optics and Image Science, and Vision