2-heptylcyclopropane-1-carboxylic acid loaded acylated chitosan membranes inhibit bacterial biofilms
Statement of Purpose: Biofilms formed on the surface of implanted medical devices and musculoskeletal wounds can cause infectious complications. Preventive anti-infective strategies for inhibition and dispersal of biofilms include local drug delivery of antimicrobials facilitated by sustained release of drugs. Guided regeneration membranes can provide a template for healing tissue and delivering antimicrobials for use as wound dressings for traumatically injured tissues. Chitosan has been investigated as a sustained drug delivery system due to its biocompatibility and physiochemical characteristics . Previous research has shown that cis-2-decenoic acid (C2DA), a short chain fatty acid, disperses and inhibits biofilm formation . However, the cis structure of C2DA is not stable at elevated temperatures or when exposed to light and radiation, due to cis/trans isomerization and oxidative degradation of its cis double bond. 2-Heptylcyclopropane-1-carboxylic acid (2CP), however, contains a cyclopropyl isoteric replacement of the cis double bond in C2DA increasing its stability and shelf life (Figure 1). Initial preliminary experiments have shown that 2-CP exhibits greater biofilm dispersion compared to C2DA. The objectives of this study were to determine loading, release, and efficacy of 2-CP loaded into acyl-modified electrospun chitosan membranes. Methods: Chitosan (Primex; molecular weight 311.5.
Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Awais, R., Harrison, Z., Raji, B., Murali, V., Bumgardner, J., Baker, D., & Jennings, J. (2019). 2-heptylcyclopropane-1-carboxylic acid loaded acylated chitosan membranes inhibit bacterial biofilms. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, 40, 778. Retrieved from https://digitalcommons.memphis.edu/facpubs/1521