A designed functional metalloenzyme that reduces O 2 to H 2O with over one thousand turnovers


No spare Tyr: Rational design of functional enzymes with a high number of turnovers is a challenge, especially those with a complex active site, such as respiratory oxidases. Introducing two His and one Tyr residues into myoglobin resulted in enzymes that reduce O 2 to H 2O with more than 1000 turnovers (red line, see scheme) and minimal release of reactive oxygen species. The positioning of the Tyr residue is critical for activity. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Publication Title

Angewandte Chemie - International Edition