Gold nanoparticles: Catalyst for the oxidation of NADH to NAD+
Nicotinamide adenine dinucleotide is an important coenzyme involved in the production of ATP, the fuel of energy, in every cell. It alternates between the oxidized form NAD+ and the reduced form dihydronicotinamide adenine dinucleotide (NADH) and serves as a hydrogen and electron carrier in the cellular respiratory processes. In the present work, the catalytic effect of gold nanoparticles on the oxidization of NADH to NAD+ was investigated. The addition of gold nanoparticles was found to quench the NADH fluorescence intensities but had no effect on the fluorescence lifetime. This suggested that the fluorescence quenching was not due to coupling with the excited state, but due to changing the ground state of NADH. The intensity of the 340 nm absorption band of NADH was found to decrease while that of the 260 nm band of NAD+ was found to increase as the concentration of gold nanoparticles increased. This conversion reaction was further supported by nuclear magnetic resonance and mass spectroscopy. The effect of the addition of NADH was found to slightly red shift and increase the intensity of the surface plasmon absorption band of gold nanoparticles at 520 nm. This gives a strong support that the conversion of NADH to NAD+ is occurring on the surface of the gold nanoparticles, i.e. NADH is surface catalyzed by the gold nanoparticles. The catalytic property of this important reaction might have important future applications in biological and medical fields. © 2005 Elsevier B.V. All rights reserved.
Journal of Photochemistry and Photobiology B: Biology
Huang, X., El-Sayed, I., Yi, X., & El-Sayed, M. (2005). Gold nanoparticles: Catalyst for the oxidation of NADH to NAD+. Journal of Photochemistry and Photobiology B: Biology, 81 (2), 76-83. https://doi.org/10.1016/j.jphotobiol.2005.05.010