Microsomal reduction of 3-amino-1,2,4-benzotriazine 1,4-dioxide to a free radical
The drug SR 4233 (3-amino-1,2,4-benzotriazine 1,4-dioxide) is under pharmacological study as the lead compound in a new series of hypoxia-activated drugs, the benzotriazine N-oxides. However, the stable two- and four-electron-reduced metabolites of SR 4233, formed by the successive loss of the two oxygen atoms, are not pharmacologically active. In order to evaluate the possibility of an initial one-electron intermediate as the active species, we have used microsomal reduction and EPR spectroscopy to identify the first free radical reduction product. The unpaired electron is primarily centered on the 1-nitrogen, and the radical is best described as a nitroxide. Results with spin-trapping experiments show that reduction of SR 4233 to a free radical is followed by its air oxidation, resulting in the formation of the superoxide radical. Experiments with specific inhibitors suggest that the drug is being reduced by microsomal NADPH-cytochrome P-450 reductase.
Lloyd, R., Duling, D., Rumyantseva, G., Mason, R., & Bridson, P. (1991). Microsomal reduction of 3-amino-1,2,4-benzotriazine 1,4-dioxide to a free radical. Molecular Pharmacology, 40 (3), 440-445. Retrieved from https://digitalcommons.memphis.edu/facpubs/1948