Structural characterization of an LPA1 second extracellular loop mimetic with a self-assembling coiled-coil folding constraint
G protein-coupled receptor (GPCR) structures are of interest as a means to understand biological signal transduction and as tools for therapeutic discovery. The growing number of GPCR crystal structures demonstrates that the extracellular loops (EL) connecting the membrane-spanning helices show tremendous structural variability relative to the more structurally-conserved seven transmembrane α-helical domains. The EL of the LPA1 receptor have not yet been conclusively resolved, and bear limited sequence identity to known structures. This study involved development of a peptide to characterize the intrinsic structure of the LPA1 GPCR second EL. The loop was embedded between two helices that assemble into a coiled-coil, which served as a receptor-mimetic folding constraint (LPA1-CC-EL2 peptide). The ensemble of structures from multi-dimensional NMR experiments demonstrated that a robust coiled-coil formed without noticeable deformation due to the EL2 sequence. In contrast, the EL2 sequence showed well-defined structure only near its C-terminal residues. The NMR ensemble was combined with a computational model of the LPA1 receptor that had previously been validated. The resulting hybrid models were evaluated using docking. Nine different hybrid models interacted with LPA 18:1 as expected, based on prior mutagenesis studies, and one was additionally consistent with antagonist affinity trends. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
International Journal of Molecular Sciences
Young, J., Clayton, B., Kikonyogo, A., Pham, T., & Parrill, A. (2013). Structural characterization of an LPA1 second extracellular loop mimetic with a self-assembling coiled-coil folding constraint. International Journal of Molecular Sciences, 14 (2), 2788-2807. https://doi.org/10.3390/ijms14022788