Title

Aldehyde oxidase activity during development in Musca domestica

Abstract

1. 1. Polyacrylamide gel electrophoresis was used to demonstrate several bands of aldehyde oxidase activity in homogenates of Musca domestica eggs, larvae, pupae, and adults. Isoelectric points of these bands were within the range of 4.4-5.3. 2. 2. Homogenates of late pupae and early adult flies exhibited the greatest aldehyde oxidase specific activities. 3. 3. Larval gut homogenate contained the greatest specific activity while Malpighian tubules and fat body homogenates, respectively, exhibited lesser aldehyde oxidase specific activities. 4. 4. Thermostability of aldehyde oxidase activity was examined by heating homogenates of larvae, pupae, and adults at 60°C for varying times. In larval and pupal homogenates approx. 70% of the aldehyde oxidase specific activity remained after heating for 1 hr whereas 80% of the adult aldehyde oxidase activity was lost after 10 min of heating. © 1982.

Publication Title

Comparative Biochemistry and Physiology -- Part B: Biochemistry and

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