Characterization of actin isoforms in ejaculated boar spermatozoa
Actin was localized in boar ejaculated spermatozoa by using specific antisera against cytoplasmic isoforms of actin [Otey et al., J Cell Biol, 102:1726–1737, 1986; Skalli et al., J Cell Biol, 103:2787–2796, 1986; Miller et al., Biochemistry, 26:6064–6070, 1987]. Indirect immunofluorescence and immunoelectron microscopy showed that γ and β actins were codistributed in the acrosomal and postacrosomal regions. Sperm actin was also identified on two‐dimensional gel as two spots in the isoelectric point and molecular weight corresponding to β and γ actins. Coelectrophoresis of sperm actin and chicken gizzard actin and immunoblots stained with the specific antibodies confirmed the presence of these two isoforms of actin. Copyright © 1988 Alan R. Liss, Inc.
Casale, A., Camatini, M., Skalli, O., & Gabbiani, G. (1988). Characterization of actin isoforms in ejaculated boar spermatozoa. Gamete Research, 20 (2), 133-144. https://doi.org/10.1002/mrd.1120200204