Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA


We investigated the replication of the region where transcription terminates in mouse rDNA. It contains a replication fork barrier (RFB) that behaves in a polar manner, arresting only replication forks moving in the direction opposite to transcription. This RFB consists of several closely spaced fork arrest sites that co-localize with the transcription terminator elements, known as Sal boxes. Sal boxes are the target for mTTF-I (murine transcription termination factor T). These results suggest that both termination of rRNA transcription and replication fork arrest may share cis-acting as well as trans-acting factors.

Publication Title

Journal of Molecular Biology