Cytokinins in Azotobacter vinelandii culture medium
Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. The total cytokinin activity was equivalent to 0.75 μg of kinetin per liter. © 1989, American Society for Microbiology.
Applied and Environmental Microbiology
Taller, B., & Wong, T. (1989). Cytokinins in Azotobacter vinelandii culture medium. Applied and Environmental Microbiology, 55 (1), 266-267. https://doi.org/10.1128/aem.55.1.266-267.1989