Interaction of juvenile hormone III with cricket (Gryllus domesticus) ovary in vitro as assessed by high performance liquid chromatography


1. 1. A study of the interaction of 3H-juvenile hormone III (JH III) with ovarian tissue of the cricket, Gryllus domesticus, was carried out in vitro. 2. 2. High performance liquid chromatography (HPLC) was used to follow the radiolabel in both media and tissue extracts. 3. 3. At 0.5, 2, 4, 8 and 12 hr incubation, tissue extracts contained low amounts (i.e., 10% or less of the total) of the radiolabel while the media extracts contained decreasing amounts of intact JH III and increasing amounts of radiolabeled metabolites. 4. 4. The conversion of JH III to polar metabolites was time- and tissue-dependent. 5. 5. The major metabolite did not co-migrate with JH acid, JH diol or JH acid-diol; but may represent a conjugated form of the JH acid-diol. 6. 6. No significant conversion occurred in the presence of large (ovulated) terminal oocytes which lacked follicle wall components. © 1987.

Publication Title

Comparative Biochemistry and Physiology -- Part B: Biochemistry and