Light localization properties of weakly disordered optical media using confocal microscopy: Application to cancer detection
We have developed a novel technique to quantify submicron scale mass density fluctuations in weakly disordered heterogeneous optical media using confocal fluorescence microscopy. Our method is based on the numerical evaluation of the light localization properties of an 'optical lattice' constructed from the pixel intensity distributions of images obtained with confocal fluorescence microscopy. Here we demonstrate that the technique reveals differences in the mass density fluctuations of the fluorescently labeled molecules between normal and cancer cells, and that it has the potential to quantify the degree of malignancy of cancer cells. Potential applications of the technique to other disease situations or characterizing disordered samples are also discussed.
Sahay, P., Almabadi, H., Ghimire, H., & Skalli, O. (2017). Light localization properties of weakly disordered optical media using confocal microscopy: Application to cancer detection. Optics Express, 25 (13), 15428-15440. https://doi.org/10.1364/OE.25.015428