Title

Localization of the sodium-potassium-chloride cotransporter (SLc12A2) during zebrafish embryogenesis and myogenesis and a screen for additional antibodies to study zebrafish myogenesis

Abstract

The objective of the current study was to screen a series of commercially avaliable antibodies in order to determine if they would bind specifically to target proteins in zebrafish musculature for use in fluorscence confocal microscopy. Many of these antibodies were not developed specifically for use in zebrafish; but because of the near universality of many basic muscle proteins among species these antibodies could potentially bind to homologus proteins in zebrafish. Of these, T4, for the protein Slc12a, has not been well described. We reviewed antibody expression in the muscle and evaluated the protein localization of Slc12a in zebrafish development. T4 was visualized within the skeletal muscle, where it may play an important role in ion regulation during muscle activity. Overall, the use of these antibodies will allow researchers access to tools known to work within the zebrafish and enable more studies using this organism as a model system for muscle development.

Publication Title

Zebrafish: Topics in Reproduction, Toxicology and Development

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