Low speed preparation of microsomes: A comparative study
Microsomal fractions were isolated from hepatic tissue, housefly abdomen and southern armyworm midgut, either by centrifugation of post-mitochondrial supernatant for 1 h at 105 000×g or by calcium sedimentation and low speed centrifugation. A comparison of the two methods of isolation were made on the basis of: NADPH oxidase activity, O-demethylation of p-nitroanisole, spectral parameters of cytochrome P-450, and integrity of ultrastructural constituents. The method of isolation did not effect these parameters appreciably for microsomes isolated from hepatic tissue. Calcium sedimentation of insect microsomes resulted in a reduction of enzyme activity and an apparent decrease in cytochrome P-450. Ultrastructural integrity did not appear to be influenced by the method of sedimentation. © 1973.
Baker, R., Coons, L., & Hodgson, E. (1973). Low speed preparation of microsomes: A comparative study. Chemico-Biological Interactions, 6 (5), 307-316. https://doi.org/10.1016/0009-2797(73)90045-8