Interleukin-10 and Th2 cytokines differentially regulate osteopontin expression in human monocytes and dendritic cells
Osteopontin (OPN) is a pleiotrophic phosphoprotein involved in homeostatic and pathophysiologic responses. It is known to be a chemotactic cytokine for dendritic cells (DCs), a critical cell type in both innate and adaptive immune responses. We report herein a contrasting role of interleukin-10 (IL-10) and Th2 cytokines in the regulation of OPN expression in human monocytes and monocyte-derived DCs (Mo-DCs). Our results showed first that the expression of OPN in monocytes and Mo-DCs was induced in a time-dependent and dose-dependent manner by IL-10 but was inhibited by IL-4 or IL-13. Further, the basal level of OPN expression was also inhibited by IL-4. This inhibitory effect of IL-4 was associated with a faster decay of OPN transcripts and a decreased proximal promoter activity of OPN in IL-4-treated cells. These results demonstrate a novel role of IL-10 and Th2 cytokines in the regulation of DC function through their contrasting regulatory activities on the expression of OPN. © Mary Ann Liebert, Inc.
Journal of Interferon and Cytokine Research
Konno, S., Eckman, J., Plunkett, B., Li, X., Berman, J., Schroeder, J., & Huang, S. (2006). Interleukin-10 and Th2 cytokines differentially regulate osteopontin expression in human monocytes and dendritic cells. Journal of Interferon and Cytokine Research, 26 (8), 562-567. https://doi.org/10.1089/jir.2006.26.562