Electronic Theses and Dissertations

Jermiah Tate

Date

2024

Document Type

Thesis

Degree Name

Master of Science

Department

Biomedical Engineering

Committee Chair

J. Amber Jennings

Committee Member

J. Amber Jennings

Committee Member

Joel D. Bumgardner

Committee Member

Tomoko Fujiwara

Abstract

Urinary catheters made of polyvinyl chloride (PVC) are susceptible to microbial biofilm formation. 10-hydroxy-2-decenoic acid (10-H2DA) is a dispersal signaling molecule that inhibits biofilm and planktonic growth of microorganisms. This study hypothesized that coating PVC with 10-H2DA would inhibit planktonic and biofilm-associated Candida albicans. Interactions of the PVC and 10-H2DA molecules were analyzed using Video Contact Angle (VCA), Fourier Transform Infrared (FTIR), and High-Performance Liquid Chromatography (HPLC). Successful coating of PVC with 10-H2DA was demonstrated through a significantly lowered contact angle and FTIR peaks in regions indicative of the 10-H2DA on the surface of PVC. Elution data displays a burst release of 50% 10-H2DA within 3-6-hours. With significance, planktonic C. albicans were inhibited in the environment of PVC coated with 10-H2DA compared to uncoated PVC, although there were no significant reductions of biofilm. Immersing PVC in 10-H2DA showed short-term surface activity, which was attributed to the short timeframe of release of 10-H2DA.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to ProQuest.

Notes

Open Access

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