Investigation and Characterization of PC12 Cells Adhesion, Proliferation, and Regeneration on Aerogels as a function of Topography and Stiffness.

Identifier

6536

Author

Martina Rodriguez Sala

Date

2019

Document Type

Thesis

Degree Name

Master of Science

Major

Physics

Concentration

Materials Science

Committee Chair

Firouzeh Sabri

Committee Member

Chenhui Peng

Committee Member

M. Shah Jahan

Committee Member

Omar Skalli

Abstract

Limitations with existing methods and materials used for nerve repair shows the significant interest to keep researching new materials for nerve implants. Recent studies show the importance of material properties to cell behavior. This body of work focuses on understanding the effect of material properties on the behavior of PC12 neurons. The first part of the work attemepts to quantify the relationships between cell parameters and substrate properties. Based on this study, random topographies with surface roughness of 0.5 µm and Young’s modulus of 2MPa are the optimum substrates for neurite outgrowth in PC12 cells. The second part investigates the feasibility of creating precise patterns on aerogel susbtrates for alignment by means of plasmonic photopatterning techniques. Those results have shown a degree of alignment in neurites due to the continuous topography.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.

Recommended Citation

Rodriguez Sala, Martina, "Investigation and Characterization of PC12 Cells Adhesion, Proliferation, and Regeneration on Aerogels as a function of Topography and Stiffness." (2019). Electronic Theses and Dissertations. 2063.
https://digitalcommons.memphis.edu/etd/2063