Electronic Theses and Dissertations

Date

2021

Document Type

Thesis

Degree Name

Master of Science

Department

Nutrition

Committee Chair

Marie van der Merwe

Committee Member

Melissa Puppa

Committee Member

Richard Bloomer

Abstract

Background: Iron is an essential mineral required for multiple metabolic actions in precise concentrations to avoid oxidative toxicity. Recent evidence suggests a novel nutrient-sensitive myokine, erythroferrone, may also act as a systemic iron regulator. This pilot study aims to investigate whether the duration of regular fasting lengths prior to exercise influences erythroferrone and muscle iron uptake. Methods: C57BL6 mice were divided into 3 groups with feeding windows relative to exercise: 1) ad libitum; 2) 6 hour window immediately post-exercise; 3) 6 hour window 5 hours post-exercise. Mice were exercised 5 days per week (Monday-Friday) for 8 weeks. Quadriceps erythroferrone and transferrin receptor-1 was assessed via PCR. Soleus sections were stained with a modified Perls stain for iron content. Results: Erythroferrone and transferrin expression was not different between groups. Soleus iron content did not differ between groups. Conclusion: Fasting length prior to exercise does not seem to influence muscle iron content.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to ProQuest

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