Electronic Theses and Dissertations

Wangkuk Son

Date

2021

Document Type

Thesis

Degree Name

Master of Science

Department

Health Studies

Committee Chair

Melissa Puppa

Committee Member

Marie Merwe

Committee Member

Keith Martin

Abstract

Background: The Western diet is characterized by a high amount of n-6 PUFA and low n-3 PUFA. Due to the structural difference of composing fatty acid, Omega-3 PUFAs have beneficial effects while omega-6 PUFAs elicit adverse effects on lipid metabolism, building the foundation of metabolic syndrome and various diseases. Objective: Determine whether fat composition in an HFD affects GC-induced alterations in lipid handling by the liver, adipose tissue, and skeletal muscle. Methods: Male wild-type C57BL/6 mice were randomized into two groups: n-6 (45% fat 177.5 g lard) and n-3 (45% fat 177.5 g Menhaden oil). After 4 weeks on their diets, groups were divided to receive either daily injections of dexamethasone (3 mg/kg/day) or sterile PBS for 1 week while continuing diets. Results: Omega-3 HFD diet ameliorates adipocyte hypertrophy and hepatic fatty accumulation by involving associated lipid metabolism markers (CD36 and FABP). Conclusion: The present study's result demonstrated that the change of fat composition in HFD could beneficially alter the fatty acid accumulation, adipocyte size, and associated lipid metabolism markers.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to ProQuest

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