Electronic Theses and Dissertations



Document Type


Degree Name

Master of Science


Electrical & Computer Engineering

Committee Chair

Chrysanthe Preza

Committee Member

Aaron AR Robinson

Committee Member

Deepak DV Venugopal


In three-dimensional structured illumination microscopy (3D-SIM) where the images are taken from the object through the point spread function (PSF) of the imaging system, data acquisition can result in images taken under undesirable aberrations that contribute to a model mismatch. The inverse imaging problem in 3D-SIM has been solved using a variety of conventional model-based techniques that can be computationally intensive. Deep learning (DL) approaches, as opposed to traditional restoration methods, tackle the issue without access to the analytical model. This research aims to provide an unrolled physics-informed generative adversarial network (UPIGAN) for the reconstruction of 3D-SIM images utilizing data samples of mitochondria and lysosomes obtained from a 3D-SIM system. This design makes use of the benefits of physics knowledge in the unrolling step. Moreover, the GAN employs a Residual Channel Attention super-resolution deep neural network (DNN) in its generator architecture. The results indicate that the addition of both physics-informed unrolling and GAN incorporation yield improvements in reconstructed results compared to the regular DL approach.


Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to ProQuest


Open Access