Electronic Theses and Dissertations
Date
2023
Document Type
Thesis
Degree Name
Master of Science
Department
Nutrition
Committee Chair
Marie Van der Merwe
Committee Member
Brandt Pence
Committee Member
Edward Rosloniec
Abstract
Research has shown the metabolic role and therapeutic potential of the gut microbiome in modulating health and disease states. Microbial metabolites are known to be involved in various metabolic functions throughout the body. The purpose of this study was to determine if the microbial metabolite imidazole propionate, shown to be increased in obesity and type II diabetes, has an effect on key human T-cell functions. Two fasted blood samples were collected from 10 human subjects. PBMCs were isolated, and a subset were stimulated in the presence of ImP. After staining for cell-surface antigens and intracellular cytokines, flow cytometry analysis was conducted to assess the effect of ImP on T-cell IFNy production and proliferation. No significant effect of ImP was observed with PMA/ionomycin-induced IFNy levels or CD3/CD28/IL-2-induced proliferation of CD4+ and CD8+ T-cells. These data suggest that ImP does not significantly alter T-cell activation under the experimental conditions used.
Library Comment
Dissertation or thesis originally submitted to ProQuest
Notes
Open Access
Recommended Citation
Gubor, Chinanu, "Imidazole Propionate As A Potential Modulator of T-Cell Function" (2023). Electronic Theses and Dissertations. 3070.
https://digitalcommons.memphis.edu/etd/3070
Comments
Data is provided by the student.