Date of Award
Master of Science
Marie Van der Merwe
Research has shown the metabolic role and therapeutic potential of the gut microbiome in modulating health and disease states. Microbial metabolites are known to be involved in various metabolic functions throughout the body. The purpose of this study was to determine if the microbial metabolite imidazole propionate, shown to be increased in obesity and type II diabetes, has an effect on key human T-cell functions. Two fasted blood samples were collected from 10 human subjects. PBMCs were isolated, and a subset were stimulated in the presence of ImP. After staining for cell-surface antigens and intracellular cytokines, flow cytometry analysis was conducted to assess the effect of ImP on T-cell IFNy production and proliferation. No significant effect of ImP was observed with PMA/ionomycin-induced IFNy levels or CD3/CD28/IL-2-induced proliferation of CD4+ and CD8+ T-cells. These data suggest that ImP does not significantly alter T-cell activation under the experimental conditions used.
Dissertation or thesis originally submitted to ProQuest
Gubor, Chinanu, "Imidazole Propionate As A Potential Modulator of T-Cell Function" (2023). Electronic Theses and Dissertations. 3070.