Electronic Theses and Dissertations
Identifier
420
Date
2011
Document Type
Dissertation
Degree Name
Doctor of Philosophy
Major
Biomedical Engineering
Committee Chair
Warren Haggard
Committee Member
Joel Bumgardner
Committee Member
John Williams
Committee Member
Judith Cole
Abstract
Osteoarthritis-associated pain of the spinal disc, knee, and hip derives from degeneration of cartilagenous tissues in these joints. Traditional therapies have focused on these cartilage (and disc specific nucleus pulposus) changes as a means of treatment through tissue grafting, regenerative synthetic implants, non-regenerative space filling implants, arthroplasty, and arthrodesis. Although such approaches may seem apparent upon initial consideration of joint degeneration, tissue pathology has shown changes in the underlying bone and vascular bed precede the onset of cartilaginous changes. It is hypothesized that these changes precedent joint degeneration and as such may provide a route for early prevention. The current work proposes an injectable biomaterial-based therapy within these subchondral and cancellous bone regions as a means of preventing or reversing osteoarthritis. Two human concentrated platelet releasate-containing alginate hydrogel/beta-tricalcium phosphate composites have been developed for this potential biomaterial application. The undertaking of assessing these materials through bench-, in vitro, and ex vivo work is described herein. These studies showed the capability of the biomaterials to initiate a wound healing response in monocytes, angiogenic and differentiation behavior in immature endothelial cells, and early osteochondral differentiation in mesenchymal stem cells. These cellular activities are associated with fracture healing and endochondral bone formation, demonstrating the potential of the biomaterials to induce osseous and vascular tissue remodeling underlying osteoarthritic joints as a novel therapy for a disease with rapidly growing healthcare costs.
Library Comment
Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Recommended Citation
McCanless, Jonathan Dempsey, "In Vitro Assessment of Biomaterial-Induced Remodeling of Subchondral and Cancellous Bone for the Early Intervention of Joint Degeneration with Focus on the Spinal Disc" (2011). Electronic Theses and Dissertations. 330.
https://digitalcommons.memphis.edu/etd/330
Comments
Data is provided by the student.