Electronic Theses and Dissertations

Date

2022

Document Type

Thesis

Degree Name

Master of Science

Department

Health Studies

Committee Chair

Brandt Pence

Committee Member

Mellissa Puppa

Committee Member

Marie Van Der Merwe

Committee Member

Liza Makowski

Abstract

Many diseases are preventable through regular exercise. However, high-intensity exercise can transiently suppress immune function. Lactate can decrease inflammatory responses through epigenetic modifications such as histone lactylation, inhibiting inflammatory genes while promoting anti-inflammatory genes. Human blood monocytes were isolated from 10 healthy males and females ranging from 23to 37 years old and pre-treated with either media or sodium lactate (10mM) for 6 hrs. An additional experiment tested if the flavonoid quercetin was able to decrease lactates’ immunosuppressive effects. Extracellular acidification rate (ECAR), oxygen consumption rate (OCR), lactyllysine levels were measured as well as expression of IL10 and TNF. ECAR and TNF were suppressed in lactate-treated monocytes while OCR and IL10 were increased. Quercetin results were not significant. Western blots showed an increase in lactylation in lactate-treated groups. Follow-up research will examine cellular signaling mechanisms as well as validate this in an exercise model.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to ProQuest.

Notes

Open access

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