Electronic Theses and Dissertations

Date

2024

Document Type

Thesis

Degree Name

Master of Science

Department

Nutrition

Committee Chair

Marie van der Merwe

Committee Member

Melissa Puppa

Committee Member

Amandeep Bajwa

Abstract

Objective: To determine if weekly mitochondrial transplants given to animals undergoing a muscle damaging protocol will improve exercise performance and immune responses. Methods: Mitochondrial injections were given to eight-week-old C57/BL6 mice (N=7/group) for 6 weeks under a muscle damaging exercise protocol. A run-to-fatigue test was completed at baseline, 4, and 6 weeks. Blood was collected immediately after the last run; tissues and organs were harvested 24 hours later for transcription measurements and histology. Results: No significant differences were found in exercise performance in any week. Histological analysis did not show any differences between groups. PGC1-α transcripts were significantly decreased in liver in the transplanted group (p = 0.01). Inflammatory cytokine IL-6 was significantly decreased after exercise (p = 0.03). Lipopolysaccharide stimulated splenocytes showed TNF-α was significantly reduced in transplanted mice. Conclusion: Mitochondrial transplant therapy does not affect exercise performance, but it does affect inflammatory responses, specifically IL-6 and TNF-α.

Comments

Data is provided by the student.”

Library Comment

Dissertation or thesis originally submitted to ProQuest.

Notes

Open Access

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