ATOMIC-FORCE-MICROSCOPE INDENTATION OF ALVEOLAR EPITHELIAL TYPE II CELLS

Identifier

7

Author

Catherine Eileen Cozad

Date

2010

Document Type

Thesis

Degree Name

Master of Science

Major

Biomedical Engineering

Committee Chair

Esra Roan

Committee Member

Chris Waters

Committee Member

Eugene Eckstein

Abstract

Cozad, Catherine Eileen. M.S. The University of Memphis. May 2010. Atomic-Force-Microscope Indentation of Alveolar Epithelial Type II Cells. Major Professor: Esra Roan, Ph.D. Primary rat alveolar epithelial type II (ATII) cells dedifferentiate over time in culture into alveolar epithelial type I (ATI) cells. The mechanical characteristics of ATII cells measured as elastic modulus (E) could be time dependent in culture. An atomic force microscope was used to perform force indentation (F-δ) on the ATII cells, using pyramidal cantilevers with spring constant of 0.03 ± 0.01 N/m. Seven isolations of ATII cells were cultured; square regions inconfluent cultureswere studied by indentation on a one micrometer gridand estimatedto touch 3-6 cells. E values were determined from F-δ curves.Some maps of Ehadindicated regions of high and low values, presumably reflecting regions with and without nucleus. Median E value was 5.93 (Day 2) and 8.37 kPa (Day 3); significant difference was determined by a non-parametric test, p ≤ 0.001, indicating that mechanical properties of ATII cells could exhibit time dependence.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.

Recommended Citation

Cozad, Catherine Eileen, "ATOMIC-FORCE-MICROSCOPE INDENTATION OF ALVEOLAR EPITHELIAL TYPE II CELLS" (2010). Electronic Theses and Dissertations. 6.
https://digitalcommons.memphis.edu/etd/6