Date of Award
Master of Science
Cozad, Catherine Eileen. M.S. The University of Memphis. May 2010. Atomic-Force-Microscope Indentation of Alveolar Epithelial Type II Cells. Major Professor: Esra Roan, Ph.D. Primary rat alveolar epithelial type II (ATII) cells dedifferentiate over time in culture into alveolar epithelial type I (ATI) cells. The mechanical characteristics of ATII cells measured as elastic modulus (E) could be time dependent in culture. An atomic force microscope was used to perform force indentation (F-δ) on the ATII cells, using pyramidal cantilevers with spring constant of 0.03 ± 0.01 N/m. Seven isolations of ATII cells were cultured; square regions inconfluent cultureswere studied by indentation on a one micrometer gridand estimatedto touch 3-6 cells. E values were determined from F-δ curves.Some maps of Ehadindicated regions of high and low values, presumably reflecting regions with and without nucleus. Median E value was 5.93 (Day 2) and 8.37 kPa (Day 3); significant difference was determined by a non-parametric test, p ≤ 0.001, indicating that mechanical properties of ATII cells could exhibit time dependence.
dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.
Cozad, Catherine Eileen, "ATOMIC-FORCE-MICROSCOPE INDENTATION OF ALVEOLAR EPITHELIAL TYPE II CELLS" (2010). Electronic Theses and Dissertations. 6.