Electronic Theses and Dissertations

Identifier

925

Date

2013

Document Type

Thesis

Degree Name

Master of Science

Major

Biomedical Engineering

Committee Chair

Warren Haggard

Committee Member

Richard Smith

Committee Member

Eugene Eckstein

Committee Member

Joel Bumgardner

Abstract

This research is the application of cyclic strain to a polyester terepthalate (PET) scaffold seeded with fibroblast cells on one half and osteoblast cells on the other half for 28 days. The apparatus ensured that osteoblasts received no applied strain except that from the flowing media. Fibroblasts received strain (0%, 2.5%, and 5%) in the form of 0.089 Hz load cycles, which incorporated 10 seconds of rest, 4 hours per day. This preliminary research showed that fibroblasts can respond to strain application and media flow by depositing approximately 2 times more glycosaminoglycans and collagen matrix onto the PET scaffold per cell (μg/μg) in comparison to a static condition. In addition, osteoblast cells produced osteocalcin, signifying that the bioreactor environment that incorporated media flow and/or cyclic strain to the fibroblasts promoted osteoblast maturation.

Comments

Data is provided by the student.

Library Comment

Dissertation or thesis originally submitted to the local University of Memphis Electronic Theses & dissertation (ETD) Repository.

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