Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

Authors

• Bo Wang, Medical College of Wisconsin
• Mickey Shah, College of Engineering and Polymer Science
• Lakiesha N. Williams, Herbert Wertheim College of Engineering
• Amy L. de Jongh Curry, Herff College of Engineering
• Yi Hong, College of Engineering
• Ge Zhang, College of Engineering and Polymer Science
• Jun Liao, College of Engineering

Abstract

Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit to myocardial infarction (MI) treatment. Here, we described an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae, myocardial slices as a biomimetic cell culture and delivery platform, and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulations for facilitating cardiac construct development.

Publication Title

Methods in Molecular Biology

Recommended Citation

Wang, B., Shah, M., Williams, L., de Jongh Curry, A., Hong, Y., Zhang, G., & Liao, J. (2022). Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct. Methods in Molecular Biology, 2485, 55-70. https://doi.org/10.1007/978-1-0716-2261-2_4