Electroanalytical and biocompatibility studies on microfabricated array sensors

Ernô Lindner, Institute for General and Analytical Chemistry
Vasile V. Cosofret, The University of North Carolina at Chapel Hill
Richard P. Buck, The University of North Carolina at Chapel Hill
Timothy A. Johnson, The University of North Carolina at Chapel Hill
R. Bruce Ash, NC State University
Michael R. Neuman, Case Western Reserve University
Weiyuan J. Kao, Case Western Reserve University
James M. Anderson, Case Western Reserve University

Abstract

pH, potassium and calcium ion‐selective membranes based on neutral ionophores TDDA, valinomycin and ETH‐1001, respectively and immobilized in high molecular weight (HMW) and carboxylated poly(viny1 chloride) (PVC‐COOH) as well as in aliphatic polyurethane (Tecoflex) matrices with normal and reduced amounts of plasticizers were investigated with respect of their general analytical properties, biocompatibility and cellular responses. The analytical performance of the PVC‐COOH based potassium electrodes was not effected by reducing the plasticizer content from 66% (w/w) to 33% (w/w) while that of pH electrodes was significantly deteriorated at the lower plasticizer level. High molecular weight PVC and Tecoflex membranes with low plasticizer content induce less inflammatory response compared to membranes cast with high plasticizer concentrations or PVC‐COOH as matrix. o‐NPOE plasticized membranes are correlated with an increased acute, but a decireased chronic inflammatory response, when compared to membranes cast with DOS as plasticizer. Copyright © 1995 VCH Verlagsgesellschaft mbH