A new HPTLC densitometric method for analysis of swertiamarin in Enicostemma littorale and commercial formulations
Abstract
A high-performance thin layer chromatographic densitometric method for the analysis of swertiamarin in 60% methanolic extract of Enicostemma littorale and commercial formulations has been developed and validated in this study. Swertiamarin was separated on aluminium-backed silica gel 60 F254 plates using ethyl acetate : methanol : water (77 : 15 : 8)% v/v as the mobile phase. This system was found to give a compact spot of swertiamarin at Rf value 0.36±0.01. The limit of detection and limit of quantification were found to be 31.25 and 103.12 ng spot-1, respectively. The proposed method was employed with a high degree of precision and accuracy for the estimation of swertiamarin in methanolic extract of Enicostemma littorale and in commercial formulations. © 2011 Taylor & Francis.
Publication Title
Natural Product Research
Recommended Citation
Alam, P., Ali, M., Singh, R., & Shakeel, F. (2011). A new HPTLC densitometric method for analysis of swertiamarin in Enicostemma littorale and commercial formulations. Natural Product Research (1), 17-25. https://doi.org/10.1080/14786411003754348
